1. Reconstructing Integrin Activation in vitro
Alexandre R. Gingras, Feng Ye, and Mark H. Ginsberg
2. Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy
Jens Friedrichs, Carsten Werner, and Daniel J. Muller
3. A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics
Warwick S. Nesbitt, Francisco J. Tovar-Lopez, Erik Westein, Ian S. Harper, and Shaun P. Jackson
4. Localization-Based Super-Resolution Imaging of Cellular Structures
Pakorn Kanachanwong and Clare M. Waterman
5. Use of Microarray Analysis to Investigate EMT Gene Signatures
Andy H. Sims, A.A. Larionov, D.J. Harrison, and Elad Katz
6. Podosome Reformation in Macrophages: Assays and Analysis
Pasquale Cervero, Linda Panzer, and Stefan Linder
7. Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells
Mirjam Andreasson-Ochsner and Erik Reimhult
8. Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay
Marie Schoumacher, Alexandros Glentis, Vasily V. Gurchenkov, and Danijela M. Vignjevic
9. Cytoplasmic Actin: Purification and Single Molecule Assembly Assays
Scott D. Hansen, J. Bradley Zuchero, and R. Dyche Mullins
10. Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity
Aron Parekh, Jonathan M. Page, Scott A. Guelcher, and Alissa M. Weaver
11. Determining Rho GTPase Activity by an Affinity-Precipitation Assay
Narendra Suryavanshi and Anne J. Ridley
12. Proteomic and Biochemical Methods to Study the Cytoskeletome
Richard L. Klemke, Xinning Jiang, Sunkyu Choi, and Jonathan A. Kelber
13. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins
Molly Lowndes and W. James Nelson
14. Purification of Native Arp2/3 Complex from Bovine Thymus
Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
15. Purification of Arp2/3 Complex from Saccharomyces Cerevisiae
Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
16. Measurement and Analysis of in vitro Actin Polymerization
Lynda K. Doolittle, Michael K. Rosen, and Shae B. Padrick
17. Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions
Simon Scrace, Eric O'Neill, Ester M. Hammond and Isabel M. Pires
18. Measuring Chemotaxis Using Direct Visualisation Microscope Chambers
Andrew J Muinonen-Martin, David A. Knecht, Douwe M. Veltman, Peter A. Thomason, Gabriela Kalna, and Robert H. Insall
19. In Vitro Microtubule Severing Assays
Natasza E. Ziólkowska and Antonina Roll-Mecak
20. VE-Cadherin Status as an Indicator of Microvascular Permeability
Elizabeth Monaghan-Benson and Keith Burridge
21. High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis
Ved P. Sharma, David Entenberg, and John Condeelis
22. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells
Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, and Louis Hodgson
23. Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion
Joshua S. McLane, Nicholas J. Schaub, Ryan J. Gilbert, and Lee A. Ligon
24. Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions
Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap
