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Исследование осмотических стрессов на &#1078

Исследование осмотических стрессов на ж

          
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About the Book

После предыдущего изучения клеточных реакций бактерий на тепловой шок и окислительный стресс, настоящее исследование было направлено на изучение влияния комбинированного воздействия высокой температуры (44°C - 46°C) и высокой концентрации сахара (декстрозы и сахарозы) на рост и размножение Saccharomyces cerevisiae SUBSC01. Рост клеток измеряли с использованием традиционного подхода оценки оптической плотности при 600 нм (OD600) и подсчета колониеобразующих единиц (КОЕ) на агаровых пластинах до 450 минут. Последующие изменения в морфологии дрожжей и расположении клеток были замечены, а сохранение жизнеспособности клеток в стрессовых условиях было подтверждено точечными тестами. Полное замедление роста наблюдалось при 45 °C при высоких концентрациях декстрозы (3,6×104 мкг/мл) и сахарозы (1,8×105 мкг/мл). Примечательно, что почкование дрожжей наблюдалось при 44 °C и 45 °C до 270 минут инкубации, которое было подавлено при 46 °C. В целом, текущее исследование продемонстрировало стрессовый ответ в дрожжевых клетках на фенотипическом уровне, что согласуется с существующими знаниями о событиях осмотического стрессового ответа у дрожжей.


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Product Details
  • ISBN-13: 9786205720202
  • Publisher: KS Omniscriptum Publishing
  • Publisher Imprint: Sciencia Scripts
  • Height: 229 mm
  • No of Pages: 52
  • Spine Width: 3 mm
  • Width: 152 mm
  • ISBN-10: 6205720205
  • Publisher Date: 20 Feb 2023
  • Binding: Paperback
  • Language: Russian
  • Returnable: N
  • Weight: 91 gr


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Исследование осмотических стрессов на &#1078
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